&nbs= p; &= nbsp; &nbs= p; &= nbsp; &nbs= p; &= nbsp; &nbs= p; &= nbsp; Allina Laboratories

&nbs= p; &= nbsp; &nbs= p; &= nbsp; &nbs= p; &= nbsp; &nbs= p;

FUNGUS/PNEUMOCYSTIS STAIN <= o:p>

Epic<= span style=3D'font-size:11.0pt;mso-bidi-font-weight:bold'> CYTOLOGY/LAB6301 Star<= /span> 3996

In Epic indicate : "Cytology Only, Cytology and Fungus, or Fungus Stain Only&quo= t;

(For Fungus Cultures/K= OH Prep see Microbiology Section)

DEPARTMENT: = ; Cytology

CONTAINER: = Sterile container labeled with a minimum of two patient identifiers, and source.

INSTRUCTIONS: Specimen Collection –SPECIFY SOURCE. Bring to lab immediately after collection.

1.&n= bsp;       Body Fl= uid:

a.     Place specimen into a labeled sterile co= ntainer.

b.     Add 500-1000 units of heparin to the container for every 100cc of specimen

        (a= s it is being collected – mixing constantly, but gently).

2.      = = Bronchial/Esophageal B= rushing:

a.      = If cultures are ordered: place brush in labeled capped tube containing sterile saline.

b.&n= bsp;      If no cultures are ordered, place brush into= a labeled tube of Cytolyt.

<= span style=3D'font-size:10.0pt;mso-bidi-font-size:12.0pt'>3.      = = Bronchial Washing/BAL (Bronchoalveolar Lavage):

                        Collect specimen into a labeled sterile mucus trap.

<= span style=3D'font-size:10.0pt;mso-bidi-font-size:12.0pt'>4.      = = Purulent Material:
                        Place specimen into a labeled sterile container or use culture swab.

            =             I= ndicate source.

5.      = = Sputum<= span style=3D'font-size:10.0pt;mso-bidi-font-size:12.0pt'>:

a.     Collect the first early morning deep cou= gh specimen since bronchial secretions have pooled overnight.

b.     The patient should be instructed to rinse out his mouth with water only and then raise material from lungs by coughin= g as deeply as possible.

c.      = Cough into sterile labeled container. If no cultures are ordered, have p= atient cough directly into a container of labeled Cytolyt™ (obtain from pathology lab).

6.      = = Culture Swab: Indicate source.=

        7.    Skin, hair, nails, vaginal, mouth, stool: See KOH Prep in Microbiology section.

        8.    Tissue:

        &= nbsp;           &nbs= p;            &= nbsp;      a.       I= f Histology, Fungus Culture and Stain - Indicate "= Fungus Stain." on Histo requisition. Also order Microbiology.

&nbs= p;            &= nbsp;           &nbs= p;            &= nbsp; b.       If Fungus Culture and Stain (= No Histology) Use Micro requisition. In Epic also order as Cytology Fungus Stain Only.=

                c.     If Fungus Sta= in Only (No Culture and No Histology)

&nbs= p;            &= nbsp;        On requisition indicate Fungus Stain Only. In Epic order Cytology Fung= us Stain Only.

REPORTING

INTERVAL:        &= nbsp; Within 48 hours of receipt.

REFERENCE

VALUE:        &= nbsp;        No organism or hyphae identified.

SPECIMEN

REJECTION:         = Unlabeled slide or unlabeled specimen containers.&nb= sp; Improper collection.

CPT<= span style=3D'mso-spacerun:yes'> Smear 88160 = and 88312     Fluid= 88112 and 88312

Reviewed:

10/99       Linda Wennerstrom, CT (ASCP)=        2/04<= span style=3D'mso-tab-count:1'>         = Linda Wennerstrom, CT (ASCP)   &nb= sp;

2/00      = ;   Linda Wennerstrom, CT (ASCP)   &nb= sp;   2/05 Revised: Linda Wennerstrom, CT (ASCP)        &= nbsp;

2/01      &nbs= p; Linda Wennerstrom, CT (ASCP)   &nb= sp;   2/06 Revised: Linda Wennerstrom, CT (ASCP)        &= nbsp;

2/02      = ;   Linda Wennerstrom, CT (ASCP)   &nb= sp;   10/07       Linda Wennerstrom, CT (ASCP)

      &nb= sp;