Specimen should be collected from a Fasting Patient ( 4 hour Fast Preferred). If SST is drawn please transfer to screw cap vial before transport. Useful for:Differential diagnosis of hypercalcemia; used to monitor dietary deficiency or toxicity in the presence of renal disease, may be useful in investigation of some patients with clinical Vitamin D deficiency due to hereditary deficiency ofn renal 1-alpha hydroxylase.